SOX5蛋白对公鸡精子发生和精子活力的作用及其定位

doi:10.11843/j.issn.0366-6964.2018.04.008

畜牧兽医学报 ›› 2018, Vol. 49 ›› Issue (4): 718-724.doi: 10.11843/j.issn.0366-6964.2018.04.008

SOX5蛋白对公鸡精子发生和精子活力的作用及其定位

许红¹, 孙研研¹, 石雷¹, 刘一帆¹, 白皓¹, 李云雷¹, 黄子妍¹, 叶建华¹, 贾亚雄¹, 王梁², 陈继兰^1*

1. 中国农业科学院北京畜牧兽医研究所, 农业部动物遗传育种与繁殖(家禽)重点实验室, 北京 100193;
2. 北京市畜牧总站, 北京 102200

收稿日期:2017-11-01 出版日期:2018-04-23 发布日期:2018-04-23
通讯作者: 陈继兰,研究员,主要从事家禽遗传育种研究,E-mail:chen.jilan@163.com
作者简介:许红(1992-),女,辽宁大连人,硕士,主要从事家禽遗传育种研究,E-mail:1310104682@qq.com。
基金资助:
国家自然科学基金（31372304；31672406）；现代农业产业技术体系（CARS-40）；中国农业科学院科技创新工程（ASTIP-IAS04；CAAS-XTCX2016010-03）

The Effect of SOX5 Protein in Spermatogenesis and Sperm Motility Regulation and Its Expression Localization in the Testis of Roosters

XU Hong¹, SUN Yan-yan¹, SHI Lei¹, LIU Yi-fan¹, BAI Hao¹, LI Yun-lei¹, HUANG Zi-yan¹, YE Jian-hua¹, JIA Ya-xiong¹, WANG Liang², CHEN Ji-lan^1*

1. Key Laboratory of Animal Genetics, Breeding and Reproduction(Poultry) of Ministry of Agriculture, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China;
2. Beijing General Station of Animal Husbandry Service, Beijing 102200, China

Received:2017-11-01 Online:2018-04-23 Published:2018-04-23

摘要/Abstract

摘要：

本研究旨在探明SOX5蛋白在家禽睾丸发育中的表达规律，分析其在睾丸发育和精子活力调控中的潜在作用。采用RT-qPCR和Western blot技术检测0、5、15、40和60周龄北京油鸡公鸡睾丸中SOX5 mRNA和蛋白的表达，并比较其在40周龄正常公鸡与弱精子症公鸡睾丸中的表达差异，通过免疫组化技术对SOX5蛋白在睾丸组织中的表达进行定位。结果表明，SOX5 mRNA和蛋白在不同周龄公鸡的睾丸中均有表达，且差异显著（P<0.05）。SOX5 mRNA和蛋白表达量趋势一致；随着周龄的增加，其表达呈现先上升后降低的趋势：0和5周龄SOX5蛋白主要在精原细胞和支持细胞中表达；15周龄SOX5蛋白在支持细胞、各级精母细胞、圆形精子细胞和成熟精子中均高表达；40周龄表达量有所降低。SOX5基因和蛋白的表达趋势与公鸡性成熟和繁殖机能衰退的趋势一致，即性成熟前后和繁殖高峰期表达量较高，在性发育前和生精机能减退时表达量较低。此外，40周龄弱精子症公鸡睾丸中，SOX5的表达显著低于正常公鸡（P<0.05）。综上表明，SOX5的表达量与公鸡睾丸发育水平和精子活力存在一定的相关，可能对其具有重要调控作用。

Abstract:

The aim of the present study was to investigate the expression of SOX5 in the testis of chickens and identify its possible roles in testis development and sperm motility regulation. The mRNA and protein expression of SOX5 in the testis of roosters at 0,5,15,40 and 60 weeks of age were determined by RT-qPCR and Western blot, respectively. Its cellular localization in the testis was determined by immunohistochemistry. The expression of SOX5 in the testis of normal and asthenospermia roosters at 40 weeks of age was also compared. The results showed that SOX5 mRNA and protein were differentially expressed in the testis at different ages (P<0.05). The expression pattern of SOX5 protein was in accordance with the mRNA. The immunohistochemical staining revealed that SOX5 was expressed in the Sertoli cells and spermatogonia at 0 and 5 weeks of age. SOX5 was high-expressed in Sertoli cells, spermatocytes, secondary spermatocytes, round and elongated spermatids, and mature sperm in the testis at 15 weeks of age. The expression started to decrease at 40 weeks. The expression pattern of SOX5 was in accordance with the sexual development and recession of the roosters, i.e. high-expressed around the sexual maturation and reproduction peak and low-expressed before the puberty and sexual recession. In addition, the expression of SOX5 in the asthenospermia roosters was lower than the normal ones at 40 weeks of age (P<0.05). In conclusion, this study indicated that the expression of SOX5 was associated with testis development and sperm motility in chickens, suggesting its regulatory roles in these processes.

中图分类号:

S831.2

许红, 孙研研, 石雷, 刘一帆, 白皓, 李云雷, 黄子妍, 叶建华, 贾亚雄, 王梁, 陈继兰. SOX5蛋白对公鸡精子发生和精子活力的作用及其定位[J]. 畜牧兽医学报, 2018, 49(4): 718-724.

XU Hong, SUN Yan-yan, SHI Lei, LIU Yi-fan, BAI Hao, LI Yun-lei, HUANG Zi-yan, YE Jian-hua, JIA Ya-xiong, WANG Liang, CHEN Ji-lan. The Effect of SOX5 Protein in Spermatogenesis and Sperm Motility Regulation and Its Expression Localization in the Testis of Roosters[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(4): 718-724.

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SOX5蛋白对公鸡精子发生和精子活力的作用及其定位

The Effect of SOX5 Protein in Spermatogenesis and Sperm Motility Regulation and Its Expression Localization in the Testis of Roosters

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